Dexamethasone ELISA Kit

Intended Use For in-vitro research use. The Dexamethasone ELISA KIT is to be used for quantitative determination of dexamethasone levels in milk, serum and tissue. Introduction Dexamethasone is a synthetic corticosteroids. Its illegal use for growth-promoting purposes is a relatively common practice in meat cattle breeding. Principle of the assay The ELISA of dexamethasone is a competitive solid-phase assay. Serum samples or standards along with dexamethasone -horseradish peroxidase (D-HRP) enzyme conjugate and dexamethasone -antibody (D-Ab) are incubated in second antibody coated wells. After incubation, the liquid contents of the wells are decanted, and the wells are washed under running tap water for removing the unbound enzyme conjugate. The bound enzyme activity is measured by developing colored product from colorless substrate after incubation. The quantity of color developed is directly related to the bound enzyme conjugate and is inversely related to the concentration of analyte. Unknown values are determined by interpolation from the standard curve

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